Amyotrophic Lateral Sclerosis (ALS) is the most typical late-onset motor neuron dysfunction, however our present data of the molecular mechanisms and pathways underlying this illness stay elusive. This assessment (1) systematically identifies machine studying research aimed on the understanding of the genetic structure of ALS, (2) outlines the primary challenges confronted and compares the completely different approaches which were used to confront them, and (3) compares the experimental designs and outcomes produced by these approaches and describes their reproducibility by way of organic outcomes and the performances of the machine studying fashions.
The vast majority of the collected research integrated prior data of ALS into their characteristic choice approaches, and skilled their machine studying fashions utilizing genomic information mixed with different kinds of mined data together with purposeful associations, protein-protein interactions, illness/tissue-specific data, epigenetic information, and identified ALS phenotype-genotype associations. The significance of incorporating gene-gene interactions and cis-regulatory components into the experimental design of future ALS machine studying research is highlighted. Improved understanding of each the altered state of CF cells and the mechanisms of current rescue methods may reveal novel therapeutic methods.
Lastly, it’s urged that future advances within the genomic and machine studying fields will result in a greater understanding of ALS genetic structure, and allow improved personalised approaches to this and different devastating and complicated ailments. Cystic fibrosis (CF), brought on by mutations to CFTR, results in extreme and progressive lung illness. The most typical mutant, ΔF508-CFTR, undergoes proteasomal degradation, extinguishing its anion channel perform. Quite a few in vitro interventions have been recognized to partially rescue ΔF508-CFTR perform but stay poorly understood. Towards this goal, we measured transcriptional profiles of established temperature, genetic, and chemical interventions that rescue ΔF508-CFTR and in addition re-analyzed public datasets characterizing transcription in human CF vs. non-CF samples from airway and complete blood.
Chosen updates in molecular and genomic pathology of esophageal most cancers
Latest years have seen speedy advances within the discipline of molecular and genomic pathology that haven’t solely improved understanding of esophageal carcinogenesis and tumor immune setting generally but in addition have reshaped pathology follow and medical administration. On this article, we offer updates on three subjects (1) human epidermal development issue receptor 2, the primary and most necessary biomarker in focused remedy of esophageal most cancers; (2) programmed dying 1/programmed dying ligand 1, current biomarkers which have proven promise in treating each esophageal adenocarcinoma and esophageal squamous cell carcinoma; and (3) human papillomavirus involvement in esophageal carcinogenesis, one of the crucial debated subjects within the discipline, mentioned right here with a renewed understanding from current genomic and molecular information.
An epidemic of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) inflicting coronavirus ailments (C0VID-19) initially reported in Wuhan, China has quickly emerged into a world pandemic affecting thousands and thousands of individuals worldwide. Molecular detection of SARS-CoV-2 utilizing reverse transcription polymerase chain response (RT-PCR) varieties the mainstay in screening, prognosis and epidemiology of the illness. Because the virus evolves by accumulating base substitutions, mutations within the viral genome may presumably have an effect on the accuracy of RT-PCR-based detection assays.
The current availability of genomes of SARS-CoV-2 isolates motivated us to evaluate the presence and potential influence of variations in goal websites of the oligonucleotide primers and probes utilized in molecular prognosis. We catalogued a complete of 132 primer or probe sequences from literature and information obtainable within the public area. Our evaluation revealed {that a} complete of 5,862 distinctive genetic variants mapped to a minimum of one of many 132 primer or probe binding websites within the genome. A complete of 29 distinctive variants have been current in ≥ 1% of genomes from a minimum of one of many continents (Asia, Africa, Australia, Europe, North America, and South America) that mapped to 36 distinctive primers or probes binding websites.
Molecular Genomic Options Related to in Vitro Response of the NCI-60 Most cancers Cell Line Panel to Pure Merchandise
Pure merchandise stay a major supply of anti-cancer chemotherapeutics. The seek for focused medication for most cancers remedy contains consideration of pure merchandise which can present new alternatives for anti-tumor cytotoxicity as single brokers or together remedy. We examined the affiliation of molecular genomic options within the well-characterized NCI-60 most cancers cell line panel with in vitro response to remedy with 1,302 small molecules which included pure merchandise, semi-synthetic pure product derivatives, and artificial compounds primarily based on a pure product pharmacophore from the Developmental Therapeutics Program of the US Nationwide Most cancers Institute’s database.
These compounds have been obtained from a wide range of plant, marine, and microbial species. Molecular data utilized for the evaluation included expression measures for 23,059 annotated transcripts, lncRNAs, and miRNAs, and information on protein-changing single nucleotide variants in 211 cancer-related genes. We discovered associations of expression of a number of genes together with SLFN11, CYP2J2, EPHX1, GPC1, ELF3, and MGMT concerned in DNA injury restore, NOTCH members of the family, ABC and SLC transporters, and each mutations in tyrosine kinases and BRAF V600E with NCI-60 responses to particular classes of pure merchandise. Hierarchical clustering recognized teams of pure merchandise which correlated with a selected mechanism of motion.
) Ammonium sulfate (Molecular Biology Grade) |
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| CE107 | GeneOn | 5 kg | EUR 128 |
) Sodium chloride (Molecular Biology Grade) |
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| CE205 | GeneOn | 500 g | EUR 52 |
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Sodium chloride (Molecular Biology Grade) |
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| CE206 | GeneOn | 1 kg | EUR 59 |
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Sodium chloride (Molecular Biology Grade) |
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| CE207 | GeneOn | 5 kg | EUR 103 |
 Urea, suitable for molecular biology |
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| GE1210-1KG | Glentham Life Sciences | 1 kg | EUR 89 |
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Urea, suitable for molecular biology |
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| GE1210-500G | Glentham Life Sciences | 500 g | EUR 64 |
 Sucrose, GlenBiol, suitable for molecular biology |
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| GC3201-1KG | Glentham Life Sciences | 1 kg | EUR 75 |
 Ammonium chloride |
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| GK0575-100G | Glentham Life Sciences | 100 g | EUR 40 |
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Ammonium chloride |
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| GK0575-1KG | Glentham Life Sciences | 1 kg | EUR 66 |
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Ammonium chloride |
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| GK0575-250G | Glentham Life Sciences | 250 g | EUR 44 |
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Ammonium chloride |
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| GK0575-500G | Glentham Life Sciences | 500 g | EUR 52 |
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Ammonium chloride |
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| 20-abx186372 | Abbexa |
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Ammonium chloride |
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| ADB0034 | Bio Basic | 500g | EUR 58.7 |
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) BCIP (Molecular Biology Grade) |
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| CE108 | GeneOn | 250 mg | EUR 63 |
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BCIP (Molecular Biology Grade) |
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| CE109 | GeneOn | 1 g | EUR 90 |
) CHAPS (Molecular Biology Grade) |
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| CE114 | GeneOn | 1 g | EUR 55 |
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CHAPS (Molecular Biology Grade) |
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| CE115 | GeneOn | 5 g | EUR 131 |
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CHAPS (Molecular Biology Grade) |
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| CE116 | GeneOn | 25 g | EUR 410 |
) DAPI (Molecular Biology Grade) |
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| CE117 | GeneOn | 5 mg | EUR 60 |
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DAPI (Molecular Biology Grade) |
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| CE118 | GeneOn | 25 mg | EUR 133 |
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DAPI (Molecular Biology Grade) |
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| CE119 | GeneOn | 100 mg | EUR 319 |
) Dimethylsulfoxide (Molecular Biology Grade) |
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| CE120 | GeneOn | 100 ml | EUR 55 |
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Dimethylsulfoxide (Molecular Biology Grade) |
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| CE121 | GeneOn | 500 ml | EUR 92 |
) DTT (Molecular Biology Grade) |
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| CE131 | GeneOn | 5 g | EUR 78 |
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DTT (Molecular Biology Grade) |
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| CE132 | GeneOn | 10 g | EUR 111 |
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DTT (Molecular Biology Grade) |
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| CE133 | GeneOn | 25 g | EUR 203 |
) Glycine (Molecular Biology Grade) |
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| CE158 | GeneOn | 1 kg | EUR 70 |
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Glycine (Molecular Biology Grade) |
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| CE159 | GeneOn | 5 kg | EUR 190 |
) HEPES (Molecular Biology Grade) |
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| CE171 | GeneOn | 100 g | EUR 82 |
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HEPES (Molecular Biology Grade) |
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| CE172 | GeneOn | 500 g | EUR 224 |
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HEPES (Molecular Biology Grade) |
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| CE173 | GeneOn | 1 kg | EUR 354 |
) Lysozyme (Molecular Biology Grade) |
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| CE188 | GeneOn | 1 g | EUR 59 |
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Lysozyme (Molecular Biology Grade) |
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| CE189 | GeneOn | 10 g | EUR 206 |
) NAD (Molecular Biology Grade) |
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| CE196 | GeneOn | 1 g | EUR 60 |
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NAD (Molecular Biology Grade) |
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| CE197 | GeneOn | 5 g | EUR 138 |
) NBT (Molecular Biology Grade) |
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| CE209 | GeneOn | 1 g | EUR 103 |
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NBT (Molecular Biology Grade) |
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| CE210 | GeneOn | 5 g | EUR 300 |
) Tris (Molecular Biology Grade) |
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| CE237 | GeneOn | 500 g | EUR 89 |
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Tris (Molecular Biology Grade) |
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| CE238 | GeneOn | 1 kg | EUR 128 |
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Tris (Molecular Biology Grade) |
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| CE239 | GeneOn | 5 kg | EUR 446 |
) Tween20 (Molecular Biology Grade) |
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| CE242 | GeneOn | 1 l | EUR 89 |
) Water (Molecular Biology Grade) |
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| CE243 | GeneOn | 500 ml | EUR 52 |
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Water (Molecular Biology Grade) |
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| CE244 | GeneOn | 1 l | EUR 56 |
 Dodecyltrimethyl ammonium chloride |
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| DD0180 | Bio Basic | 50g | EUR 65.66 |
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) Bis-Acrylamid (Molecular Biology Grade) |
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| CE110 | GeneOn | 50 g | EUR 79 |
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Bis-Acrylamid (Molecular Biology Grade) |
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| CE111 | GeneOn | 250 g | EUR 216 |
) Formamide deionized (Molecular Biology Grade) |
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| CE145 | GeneOn | 500 ml | EUR 73 |
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Formamide deionized (Molecular Biology Grade) |
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| CE146 | GeneOn | 1 l | EUR 100 |
) Glycerol 87 % (Molecular Biology Grade) |
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| CE154 | GeneOn | 1 l | EUR 78 |
) Glycerol waterfree (Molecular Biology Grade) |
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| CE155 | GeneOn | 500 ml | EUR 65 |
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Glycerol waterfree (Molecular Biology Grade) |
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| CE156 | GeneOn | 1 l | EUR 85 |
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Glycerol waterfree (Molecular Biology Grade) |
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| CE157 | GeneOn | 2.5 l | EUR 142 |
) Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE160 | GeneOn | 100 g | EUR 78 |
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Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE161 | GeneOn | 250 g | EUR 128 |
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Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE162 | GeneOn | 500 g | EUR 194 |
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Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE163 | GeneOn | 1 kg | EUR 294 |
) Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE164 | GeneOn | 100 g | EUR 72 |
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Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE165 | GeneOn | 500 g | EUR 160 |
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Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE166 | GeneOn | 1 kg | EUR 256 |
) Urea Crystalline (Molecular Biology Grade) |
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| CE167 | GeneOn | 1 kg | EUR 60 |
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Urea Crystalline (Molecular Biology Grade) |
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| CE168 | GeneOn | 5 kg | EUR 151 |
) MOPS buffer (Molecular Biology Grade) |
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| CE194 | GeneOn | 100 g | EUR 85 |
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MOPS buffer (Molecular Biology Grade) |
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| CE195 | GeneOn | 250 g | EUR 141 |
-Sucrose (Molecular Biology Grade)) D(+)-Sucrose (Molecular Biology Grade) |
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| CE224 | GeneOn | 500 g | EUR 56 |
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D(+)-Sucrose (Molecular Biology Grade) |
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| CE225 | GeneOn | 1 kg | EUR 70 |
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D(+)-Sucrose (Molecular Biology Grade) |
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| CE226 | GeneOn | 5 kg | EUR 173 |
) Tris - Hydrochloride (Molecular Biology Grade) |
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| CE234 | GeneOn | 250 g | EUR 83 |
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Tris - Hydrochloride (Molecular Biology Grade) |
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| CE235 | GeneOn | 500 g | EUR 120 |
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Tris - Hydrochloride (Molecular Biology Grade) |
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| CE236 | GeneOn | 1 kg | EUR 186 |
) TritonX-100 (Molecular Biology Grade) |
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| CE240 | GeneOn | 500 ml | EUR 56 |
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TritonX-100 (Molecular Biology Grade) |
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| CE241 | GeneOn | 1 l | EUR 66 |
 Water, Ultrapure Molecular Biology Grade |
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| 41024-4L | Biotium | 4L | EUR 121 |
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Description: Minimum order quantity: 1 unit of 4L |
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 Tween 20, Molecular Biology Grade |
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| T9100-010 | GenDepot | 100ml | EUR 72 |
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Tween 20, Molecular Biology Grade |
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| T9100-050 | GenDepot | 500ml | EUR 111 |
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Tween 20, Molecular Biology Grade |
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| T9100-100 | GenDepot | 1L | EUR 134 |
 Water, distilled, GlenBiol™, suitable for molecular biology |
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| GK8512-1L | Glentham Life Sciences | 1 l | EUR 77 |
 Agarose, low EEO, GlenBiol, suitable for molecular biology |
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| GE6258-100G | Glentham Life Sciences | 100 g | EUR 181 |
 Double distilled for Molecular Biology) Phenol, (Carbolic acid) Double distilled for Molecular Biology |
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| PD0252 | Bio Basic | 500g | EUR 160.49 |
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ammonium chloride) benzyldimethyl(octadecyl)ammonium chloride |
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| 20-abx183877 | Abbexa |
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) EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE135 | GeneOn | 250 g | EUR 60 |
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EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE136 | GeneOn | 500 g | EUR 72 |
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EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE137 | GeneOn | 1 kg | EUR 104 |
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EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE138 | GeneOn | 5 kg | EUR 349 |
-Glucose waterfree (Molecular Biology Grade)) D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE148 | GeneOn | 500 g | EUR 56 |
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D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE149 | GeneOn | 1 kg | EUR 63 |
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D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE150 | GeneOn | 5 kg | EUR 150 |
) Yeast extract powder (Molecular Biology Grade) |
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| CE169 | GeneOn | 500 g | EUR 111 |
) Hyaluronidase Grade I (Molecular Biology Grade) |
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| CE174 | GeneOn | 1 g | EUR 194 |
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Hyaluronidase Grade I (Molecular Biology Grade) |
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| CE175 | GeneOn | 5 g | EUR 767 |
) Magnesium acetate - Tetrahydrate (Molecular Biology Grade) |
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| CE190 | GeneOn | 500 g | EUR 82 |
) NADH - Disodium salt (Molecular Biology Grade) |
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| CE198 | GeneOn | 1 g | EUR 76 |
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NADH - Disodium salt (Molecular Biology Grade) |
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| CE199 | GeneOn | 5 g | EUR 204 |
) NADP - sodium salt (Molecular Biology Grade) |
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| CE200 | GeneOn | 250 mg | EUR 77 |
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NADP - sodium salt (Molecular Biology Grade) |
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| CE201 | GeneOn | 1 g | EUR 159 |
) NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE202 | GeneOn | 25 mg | EUR 59 |
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NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE204 | GeneOn | 500 mg | EUR 312 |
 (Molecular Biology Grade)) SSC Buffer (20X) (Molecular Biology Grade) |
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| CE229 | GeneOn | 1 l | EUR 72 |
) XTT sodium salt (Molecular Biology Grade) |
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| CE250 | GeneOn | 100 mg | EUR 174 |
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XTT sodium salt (Molecular Biology Grade) |
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| CE251 | GeneOn | 500 mg | EUR 510 |
 Dodecyl dimethyl benzyl ammonium chloride |
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| 20-abx180076 | Abbexa |
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 (Molecular Biology Grade)) Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE100 | GeneOn | 50 g | EUR 107 |
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Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE101 | GeneOn | 100 g | EUR 161 |
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Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE102 | GeneOn | 250 g | EUR 323 |
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Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE103 | GeneOn | 500 g | EUR 547 |
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Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE104 | GeneOn | 1 kg | EUR 969 |
 (Molecular Biology Grade)) EDTA solution pH 8.0 (0.5 M) (Molecular Biology Grade) |
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| CE141 | GeneOn | 500 ml | EUR 73 |
) LB-Agar - Powder according to Lennox (Molecular Biology Grade) |
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| CE178 | GeneOn | 500 g | EUR 91 |
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LB-Agar - Powder according to Lennox (Molecular Biology Grade) |
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| CE179 | GeneOn | 2.5 kg | EUR 251 |
) LB-Agar - Powder according to Miller (Molecular Biology Grade) |
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| CE180 | GeneOn | 500 g | EUR 87 |
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LB-Agar - Powder according to Miller (Molecular Biology Grade) |
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| CE181 | GeneOn | 2.5 kg | EUR 246 |
) LB-Medium - Powder according to Lennox (Molecular Biology Grade) |
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| CE182 | GeneOn | 500 g | EUR 90 |
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LB-Medium - Powder according to Lennox (Molecular Biology Grade) |
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| CE183 | GeneOn | 2.5 kg | EUR 251 |
) LB-Medium - Powder according to Miller (Molecular Biology Grade) |
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| CE184 | GeneOn | 2.5 kg | EUR 246 |
 Agarose LE, Ultra-Pure Molecular Biology Grade, 100 g |
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| 41028-100G | Biotium | 100G | EUR 222 |
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Description: Minimum order quantity: 1 unit of 100G |
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 Agarose LE, Ultra-Pure Molecular Biology Grade, 25 g |
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| 41028-25G | Biotium | 25G | EUR 109 |
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Description: Minimum order quantity: 1 unit of 25G |
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 Agarose LE, Ultra-Pure Molecular Biology Grade, 500 g |
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| 41028-500G | Biotium | 500G | EUR 719 |
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Description: Minimum order quantity: 1 unit of 500G |
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 AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT |
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| AGR-LE-100 | CORNING | 1/pk | EUR 168 |
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Description: Bioscience Mol Bio; Agarose |
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, without ammonium chloride, 0.2um filtered) M9 Minimal Salts (10X), without ammonium chloride, 0.2um filtered |
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| M1035-100 | GenDepot | 1L | Ask for price |
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M9 Minimal Salts (10X), without ammonium chloride, 0.2um filtered |
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| M1035-120 | GenDepot | 20x1L | Ask for price |
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M9 Minimal Salts (10X), without ammonium chloride, 0.2um filtered |
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| M1035-150 | GenDepot | 50x1L | Ask for price |
 AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 500G/UNIT, 10 UNITS PER CASE |
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| AGR-LE-5001 | CORNING | 1/pk | EUR 3058 |
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Description: Bioscience Mol Bio; Agarose |
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 AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE |
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| AGR-MS-1001 | CORNING | 1/pk | EUR 2038 |
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Description: Bioscience Mol Bio; Agarose |
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 100 ML, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS |
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| 46-000-CI | CORNING | 100 mL/pk | EUR 57 |
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Description: Media Catalog; Sterile Wi-Fi Qual, Cell |
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 1 L, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS |
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| 46-000-CM | CORNING | 1L/pk | EUR 98 |
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Description: Media Catalog; Sterile Wi-Fi Qual, Cell |
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 500 ML, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS |
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| 46-000-CV | CORNING | 500 mL/pk | EUR 106 |
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Description: Media Catalog; Sterile Wi-Fi Qual, Cell |
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 AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT, 10 UNITS PER CASE |
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| AGR-LE-1001 | CORNING | 1/pk | EUR 772 |
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Description: Bioscience Mol Bio; Agarose |
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) AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE (AL) |
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| AGR-LM-1001 | CORNING | 1/pk | EUR 3737 |
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Description: Bioscience Mol Bio; Agarose |
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) AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 50G/UNIT, 10 UNITS PER CASE (AL) |
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| AGR-LM-501 | CORNING | 1/pk | EUR 2075 |
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Description: Bioscience Mol Bio; Agarose |
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 Ammonium Glycyrrhizinate |
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| B2155-100 | ApexBio | 100 mg | EUR 982 |
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Description: Ammonium Glycyrrhizinate |
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|
Ammonium Glycyrrhizinate |
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| B2155-25 | ApexBio | 25 mg | EUR 342 |
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Description: Ammonium Glycyrrhizinate |
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Ammonium Glycyrrhizinate |
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| B2155-5 | ApexBio | 5 mg | EUR 147 |
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Description: Ammonium Glycyrrhizinate |
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Ammonium Glycyrrhizinate |
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| B2155-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 412 |
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Description: Ammonium Glycyrrhizinate |
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 Pyrrolidinedithiocarbamate ammonium |
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| B6422-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 108 |
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Pyrrolidinedithiocarbamate ammonium |
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| B6422-50 | ApexBio | 50 mg | EUR 137 |
) Pyrrolidinedithiocarbamate (ammonium) |
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| HY-18738 | MedChemExpress | 10mM/1mL | EUR 126 |
 Ammonium bicarbonate |
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| GW0398-1KG | Glentham Life Sciences | 1 kg | EUR 54 |
Particularly, a number of pure product clusters have been related to SLFN11 gene expression, suggesting that potential motion of those compounds could contain DNA injury. The associations between gene expression or genome alterations of functionally related genes with the response of most cancers cells to pure merchandise present new details about potential mechanisms of motion of those recognized clusters of compounds with probably related organic results. This data will help in future drug discovery and in design of recent focused most cancers chemotherapy brokers.